Conference Roundup

RNA profiling explored as noninvasive diagnostic tool in melanoma

Posted on June 7, 2022

The sensitivity of RNA profiling using adhesive transparent patches (ATP) is high but specificity is low, according to a study.

In this prospective nonrandomized trial, RNA extracted from ATP was used to test for mRNA expression of PRAME and LINC00518 genes in 126 specimens. GAPDH was used as a reference gene.

Pathology reports from excisional biopsy detected melanoma in 38.9% of patients (49 out of 126); non-melanoma skin cancer in 3.2% (4 out of 126); and dysplastic nevus in 44.4% (56 out of 126).

Amplifiable mRNA was found in all ATP samples, which were taken the day of the excision. Approximately 65% (82 out of 126) of samples had positive markers.

Sensitivity for detection of skin cancers was 88.14% and specificity was 53.85%, with a positive predictive value of 63.41%, a negative predictive value of 83.33%, and accuracy of 70.16%.

There were 7 false negatives of which 3 were melanoma in situ and 4 invasive melanoma.

Samoylenko I, et al. Multicenter prospective clinical trial of molecular genetic markers for non-invasive differential diagnosis of benign and malignant melanocytic skin lesions. Presented at: ASCO 2022.